If you plan to open a document in your USB pendrive using a computer that might be breeding ground for USB viruses and malware, it would be wise to enable write-protect on the pendrive to prevent it from being infected by those threats. Enable write protect means you disable any change to be made to the files and sectors of the pendrive. There are several methods to do that but I found out using a portable write protector software is the easiest.
Portable USB WriteProtector by Werner Rumpeltesz will get the work done. It is compatible with all Microsoft Windows Operating systems up to Vista. Extract the .rar file (187kb) to the location where you want to access this software. Launch it from there.

Plug in your USB pendrive then turn on USB write protection. To disable it choose the latter.
Download Portable USB WriteProtector 1.1.0.0 | Mirror
May 28th, 2009 at 11:13 pm
At first I thought you were referring to the encrypted USB pens but then I re-read your post. I can see why you would want to protect your pen but wouldn’t it be a lot easier to just not use a possible infested computer in the first place?
May 29th, 2009 at 3:20 am
It’s difficult to tell if a computer has been infested by viruses or not unless if we do total system check on it using a reliable antivirus software with up-to-date database.
June 1st, 2009 at 9:58 am
Maybe someone sharing your files that’s why you can’t do anything about it. If you really can’t copy it on CD or on your Pendrive, try downloading a fresh ocpy of Itunes.exe.
June 2nd, 2009 at 12:46 pm
Yes I could see circumstances where this could be very useful. You might need to access something on your usb pendrive but you don’t have the time to ensure the computer is secure. I personally don’t use portable usb drives, but if I did I would want to protect it like this.
June 4th, 2009 at 10:43 am
When would you not want write-protection on, or would you ever?
-Jack
July 2nd, 2009 at 9:28 am
my laptop does support boot from usb drives, but I’m a newbie at linux, so I don’t know nothing about the exact procedure to make the pendrive bootable and how to copy the ISO file on it.
July 2nd, 2009 at 9:29 am
Windows xp and vista have a tool for this anyway,just plug in the usb drive then my computer/right click the usb drive/format/defaults or however you want to do it(defaults is easiest).
July 2nd, 2009 at 9:30 am
Make sure that the file is a iso file and then goto your bios and tell it to boot from usb disk (obviously most people have the primary boot as cd/dvd rom then hdd but you should be able to add a third or even fourth boot device.
September 1st, 2009 at 9:27 am
What os are you using?.Windows xp and vista have a tool for this anyway,just plug in the usb drive then my computer/right click the usb drive/format/defaults or however you want to do it(defaults is easiest).
September 1st, 2009 at 9:28 am
Then simply either copy or even paste the file to the usb drive.Make sure that the file is a .iso file and then goto your bios and tell it to boot from usb disk (obviously most people have the primary boot as cd/dvd rom then hdd but you should be able to add a third or even fourth boot device.
October 27th, 2009 at 3:53 am
That could have come in very handy just the other day when I managed to get a virus on my USB.
It’s all better now though
December 1st, 2009 at 9:59 am
Excellent advice. I guess my portable usb device needs a condom!
January 6th, 2010 at 11:36 pm
great article , nobody ever worries about this with their memory sticks.
January 7th, 2010 at 7:34 am
the information that it’s compatible to all type of OS is really great:)
June 2nd, 2010 at 8:23 pm
This is fantastic. Atleast now I can protect my USB drive from virus. In fact I was looking for such software.Whether it work for any PC once you write protected it at some PC.